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. 2021 Jan 28;11:2573. doi: 10.1038/s41598-021-82069-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Circadian rhythms of wheel-running activity in two extreme founder strains along with C57BL/6J strain. (A) Representative actograms of wheel-running activity in C57BL/6J, A/J and CAST/EiJ mice that were maintained under 12:12 light–dark cycles for entrainment and then released to constant darkness for free-run. (B) The period and (C) amplitude of the activity rhythms during constant darkness were quantified by periodogram analysis. Shade indicates light-off. Bar graphs depict mean ± SEM. Bar graphs with fill pattern indicate the data from males. Asterisks indicate significant differences (p < 0.05).