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. 2021 Jan 28;11:2595. doi: 10.1038/s41598-021-81587-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic analysis for identification of species of Rickettsiae based on 450 nucleotides of 17 kDa gene (A), 382 nucleotides of gltA gene (B), 540 nucleotides of rOmpA gene (C) and 426 nucleotides of rOmpB gene (D). Sequence were aligned by using MEGA5 software (https://www.megasoftware.net). Neighbor-joining phylogenetic tree construction and bootstrap analysis were performed according to the Kimura 2-parameter distances method. Bold-face font indicate positive samples detected from ticks in this study (shown only representative sample no. from among detected SFGR).