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. 2021 Jan 28;12:652. doi: 10.1038/s41467-021-20892-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Unsupervised clustering of the single-cell transcriptional profiles of FACS purified PDGFRα⁺ cells derived from hiPSCs. Visualization with a UMAP embedding divides the single cells into seven clusters. On the lower panel, co-imbedding of the hiPSC-derived data set (ipsc) with the hESC-derived PDGFR-tdTom data set from Fig. 4 shows the two data sets have overlapping cell populations. b Distribution of various genetic markers among the different clusters presented as an enrichment heatmap. All the differentially expressed genes are listed in Supplementary Data 7. c Heatmaps of GSEA show cluster-specific enrichment of OL and AS-related pathways and highlights sub-populations of OPCs that are cytokine-responsive. Detailed GSEA for all the clusters is listed in Supplementary Data 7. d GSEA enrichment plot for cytokine-mediated signaling pathway that is enriched in iP-OPC1. e Unsupervised clustering of the single-cell transcriptional profiles of D85 purified PDGFRα-tdTomato⁺ cells from an independent batch of differentiation. Peri pericytes, AS astrocytes, CyP Cycling progenitors, OL oligodendrocytes. f Co-imbedding of the D85 data set (hESC_10X) with the reference (generated by integrating snRNAseq-based transcriptome from three previously published data sets, Supplementary Fig. S4). Oligo oligodendrocytes, COP committed oligodendrocyte progenitors. g GSEA enrichment plot for cytokine-mediated signaling pathway that is enriched in D85-OPC1. h Heatmaps of GSEA for OPC sub-cluster (D85-OPC1) highlighting cytokine-mediated signaling pathway. Detailed GSEA for all the clusters is listed in Supplementary Data 6. c, h darker orange indicates higher enrichment.