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. 2021 Jan 28;11:2505. doi: 10.1038/s41598-021-82100-y

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of stressed RBCs. RBCs obtained from 8-week-old BALB/c mice were incubated at 48 °C for 30 min or kept at room temperature for 30 min (n = 9). (A,B) Cell volume of stressed RBCs and untreated RBCs. Cell volume was represented by forward scatter width. (C,D) ROS level of stressed RBCs and untreated RBCs. (E,F) Intracellular Ca²⁺ concentration of stressed RBCs and untreated RBCs. (G,H) PS externalization of stressed RBCs and untreated RBCs (*p < 0.05; **p < 0.01).