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. 2021 Jan 28;11:2505. doi: 10.1038/s41598-021-82100-y

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stressed RBCs tended to be phagocytosed. FITC-TER 119-labelled RBCs were incubated with BALB/c macrophage cell line Raw 264.7 (n = 9). (A,B) FACS results of erythrophagocytosis. Erythrophagocytosis was represented by FITC-mean fluorescence intensity. (C,F) Immunofluorescence results of erythrophagocytosis. Bright field showed macrophages treated with untreated RBCs (C) and stressed RBCs (D), fluorescence field showed TER-119 labelled untreated RBCs (E) and stressed RBCs (F). Arrow showed RBCs located in macrophages. (G) statistic results of erythrophagocytosis of untreated RBCs and stressed RBCs (*p < 0.05; **p < 0.01).