. 2021 Jan 15;7:600772. doi: 10.3389/fvets.2020.600772

Table 5.

Effect of dietary supplementation with different types of B. subtilis on health-related genes of weaned piglets.

Items	Control group	BS-A group	BS-B group	BS-C group
Day 7
IL-1β	1.00 ± 0.06^b	1.57 ± 0.31^a	1.88 ± 0.06^a	1.38 ± 0.12^a ^b
IL-2	1.00 ± 0.12^b	1.39 ± 0.04^a	1.18 ± 0.10^a ^b	1.46 ± 0.03^a
IL-6	1.00 ± 0.10^c	1.47 ± 0.12^b	1.75 ± 0.08^a	1.83 ± 0.05^a
IL-10	1.00 ± 0.17^b	1.88 ± 0.44^a ^b	1.98 ± 0.18^a	1.54 ± 0.24^a ^b
IFN-α	1.02 ± 0.12^b	1.31 ± 0.04^a	1.10 ± 0.09^a ^b	1.37 ± 0.03^a
TNF-α	1.00 ± 0.13	1.90 ± 0.47	1.68 ± 0.10	1.74 ± 0.41
TLR-4	1.00 ± 0.06^b	1.48 ± 0.06^a	1.46 ± 0.16^a	1.29 ± 0.09^a ^b
E-cadherin	1.00 ± 0.08^b	1.23 ± 0.01^a	1.32 ± 0.06^a	1.24 ± 0.01^a
Occludin	1.00 ± 0.08^b	1.66 ± 0.26^a	2.00 ± 0.14^a	1.87 ± 0.21^a
ZO-1	1.00 ± 0.08^b	1.90 ± 0.29^a	1.49 ± 0.17^a ^b	1.16 ± 0.08^b
Day 21
IL-1β	1.00 ± 0.25	1.37 ± 0.27	1.32 ± 0.06	1.33 ± 0.28
IL-2	0.69 ± 0.06^b	1.69 ± 0.29^a	1.35 ± 0.31^a ^b	1.17 ± 0.05^a ^b
IL-6	1.00 ± 0.07^b	1.38 ± 0.19^b	1.50 ± 0.12^a	1.63 ± 0.03^a
IL-10	1.00 ± 0.24	1.30 ± 0.15	1.15 ± 0.14	1.22 ± 0.11
IFN-α	1.00 ± 0.17^b	1.64 ± 0.08^a	1.65 ± 0.11^a	0.93 ± 0.07^b
TNF-α	1.00 ± 0.04^b	1.93 ± 0.35^a ^b	2.62 ± 0.50^a	1.59 ± 0.26^a ^b
TLR-4	1.00 ± 0.15	1.57 ± 0.30	1.28 ± 0.18	1.03 ± 0.07
E-cadherin	0.90 ± 0.02^b	1.37 ± 0.18^a	1.47 ± 0.07^a	1.32 ± 0.12^a
Occludin	1.00 ± 0.06	1.26 ± 0.20	1.49 ± 0.23	1.29 ± 0.12
ZO-1	1.00 ± 0.02	0.98 ± 0.07	1.17 ± 0.14	1.18 ± 0.14
Day 42
IL-1β	1.00 ± 0.15^b	3.43 ± 0.34^a	3.63 ± 0.33^a	4.12 ± 0.35^a
IL-2	1.00 ± 0.18^b	2.74 ± 0.26^a	3.50 ± 0.75^a	2.83 ± 0.46^a
IL-6	1.00 ± 0.14^b	1.09 ± 0.11^a ^b	1.42 ± 0.10^a	1.24 ± 0.12^a ^b
IL-10	1.00 ± 0.05^c	2.22 ± 0.15^b	5.07 ± 0.59^a	4.08 ± 0.50^a
IFN-α	1.00 ± 0.19	1.20 ± 0.30	1.24 ± 0.09	1.64 ± 0.23
TNF-α	1.00 ± 0.05^c	2.18 ± 0.11^b ^c	3.73 ± 0.45^a	2.81 ± 0.51^b
TLR-4	1.00 ± 0.14^b	1.83 ± 0.26^a	2.08 ± 0.15^a	2.23 ± 0.32^a
E-cadherin	1.00 ± 0.14^c	1.80 ± 0.03^a ^b	1.55 ± 0.09^b	1.93 ± 0.07^a
Occludin	1.00 ± 0.12^b	1.36 ± 0.13^a ^b	1.34 ± 0.15^a ^b	1.79 ± 0.22^a
ZO-1	1.00 ± 0.14^b	1.35 ± 0.14^a ^b	1.51 ± 0.12^a	1.41 ± 0.03^a

Data are presented as means ± SE, n = 5.

^a,b,c

Mean values within the same row with different superscript letters were significantly different (P < 0.05). IL, interleukin; IFN-α, Interferon-alpha; TLR, toll-like receptor; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1. Control group, a basal diet; BS-A group, a basal diet supplemented with B. subtilis A at 1 × 10⁶ CFU/g feed; BS-B group, a basal diet supplemented with B. subtilis B at 1 × 10⁶ CFU/g feed; and BS-C group, a basal diet supplemented with B. subtilis C at 1 × 10⁶ CFU/g feed.