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. 2021 Jan 28;11:2424. doi: 10.1038/s41598-021-81975-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reversibility of the epigenetically silenced state via epigenetic intervention through small molecule inhibition. (A) Representative microscopy images of treated DN-Late cells with either 10 µM 5-Aza-dc, 100 nM TSA, or a combination of both molecules at similar concentrations. (B) Flow cytometry comparing the expression of mNeonGreen-NLS and mRuby3-NLS within untreated DN-Late cells to DN-Late cells treated over the duration of 72hrs with a combination of 5-Aza-dc and TSA. (C) Summary of treatment conditions comparing the response of both DN-Early and DN-Late cells to a variety of inhibitory conditions using either Dimethyl Sulfoxide (DMSO), 5-Aza-dc only, TSA only, or a combination of both 5-Aza-dc and TSA. All conditions were performed in triplicate with significant changes (**p < 0.01, Bonferroni-corrected multiple t-Test) represented above.