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. 2021 Jan 28;11:2462. doi: 10.1038/s41598-021-81679-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Demonstration of miRNA-mediated gene silencing in HEK-293 cells transfected with miRNA-C plasmid constructs, determined by luminescence assay measuring CYP1A2 enzyme activity. CYP1A2 activity (%)/(pmol) was measured in absence and presence of 5 µM α-naphthoflavone, a selective CYP1A2 inhibitor. CYP1A2 enzyme activity (pmol) was calculated with a calibration curve (Fig. S3) using recombinant CYP1A2 (mean ± standard deviation; n = 3; ***P < 0.001 versus no miRNA; ^###P < 0.001 versus control).