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. 2021 Jan 28;12:650. doi: 10.1038/s41467-021-20941-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Percentages of intrahepatic CD4⁺ T cells, CD8⁺ T cells, DNT, NK cells, and NKT cells relative to the total number of CD45.2⁺ cells in mice. NCD, black; HFD, red; HFD + cDNT, blue. Actual p values (left → right): 0.028, 0.027, 0.017, 0.033, 0.016. b Absolute numbers of CD4⁺ T cells, CD8⁺ T cells, DNT, NK cells, and NKT cells in mouse livers. Actual p values (left → right): 0.00039, 0.021, 0.0039, 0.0045, 0.026, 0.0027. c Statistical analysis of the percentages of CD69⁺ cells among T cells, NK cells, and NKT cells. Actual p values (left → right): 0.000019, 0.0060, 0.0094. d Representative flow cytometry plots (top) and statistical analysis (bottom) of Annexin V⁺ cells relative to total T cells, NK cells, and NKT cells in each group. Actual p values (left → right): 0.027, 0.047, 0.0045. e, f Representative flow cytometry plots (e) and statistical analysis (f) of the percentages of IFN-γ⁺, IL-13⁺, IL-17⁺, and Foxp3⁺ cells among liver infiltrated T cells. Actual p values (left → right): f 0.00013, 0.00013, 0.000006, 0.0081, 0.015. g Real-time PCR analysis of related genes in livers from mice in each group. Actual p values (left → right): 0.015, 0.016, 0.012, 0.00041, 0.0066, 0.0029, 0.0063, 0.024, 0.000016, 0.0017. h Percentages of T cells, NK cells, and NKT cells among CD45.2⁺ cells from adipose tissue. Actual p values (left → right): 0.021, 0.034, 0.000013, 0.000006, 0.0092, 0.00042. i Relative mRNA levels of Tbx21, Gata3, Foxp3, RORγt, Ifng, and Il17 in adipose tissue. Actual p values (left → right): 0.000038, 0.00022, 0.00027, 0.022, 0.00028, 0.000026, 0.037, 0.000007, 0.0065. Data are presented as the mean ± SD; n = 6 mice/group. Statistical analysis for b of CD8 group, d of DNT group, and g of Rorgt group was performed by Kruskal–Wallis multiple comparisons test, and others were performed by one-way ANOVA with post hoc multiple comparisons test. Two-sided p values < 0.05 were considered significant. *p < 0.05; **p < 0.01. Source data, including exact p values, are provided as a Source data file.