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. 2021 Jan 28;12:650. doi: 10.1038/s41467-021-20941-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The distributions of upregulated (107) and downregulated genes (114; ≥1.5-fold difference, padj < 0.05) in cDNT treated with or without IL-10 are depicted in a volcano plot. b, c Biological process (BP) and KEGG pathway analyses were performed based on the significantly upregulated and downregulated genes in cDNT. d Heatmap showing the upregulated and downregulated genes related to cell survival and activation, cell cytotoxicity, immune responses, and signaling pathways in cDNT. e The significantly changed genes related to cell survival were confirmed via real-time PCR after IL-10 stimulation of cDNT (n = 9 biologically independent samples per group). Actual p values (left → right): 0.00026, 0.0037, 0.0028, 6.50e−7, 0.012, 0.000024, 0.0017, 0.048, 5.68e−7, 0.000081, 0.0072. f Relative expression of cell markers plotted as the fold change in expression compared to cDNT without IL-10 stimulation (n = 6 biologically independent samples per group). W/O IL-10, red; W/IL-10, blue. Actual p values (left → right): 0.027, 0.000008, 0.030. g The expression of cell cytotoxicity-related genes were determined via real-time PCR (n = 9 biologically independent samples per group). Actual p values (left → right): 0.047, 0.000076, 0.000002, 0.00019, 0000025, 00000024, 0.00015, 0.000097, 0.0031, 0.000069, 0.0078, 0.0046, 0.0038, 0.012, 0.020, 0.0011, 0.0054, 0.00022. h Representative flow cytometry plots (left) and statistical analysis (right) of NKG2A⁺ and NKG2D⁺ cells among cDNT after IL-10 stimulation (n = 5 biologically independent samples per group). Actual p values (left → right): 0.038, 0.00067, 0.000045. i cDNT incubated with anti-CD3/CD28 antibodies were pretreated with anti-IL-10R neutralizing mAb (1 and 10 μg/ml) or isotype control for 4 h, and cocultured with IL-10 (50 ng/ml) for 24 h. Statistical analysis of NKG2A⁺ cells relative to cDNT in each group (n = 4 biologically independent samples per group). Actual p values (left → right): 5.06e−7, 0.0030, 0.00028. Data are presented as the mean ± SD. Statistical analysis for f was performed by Student’s t test, and others were performed by one-way ANOVA with post hoc multiple comparisons test. Two-sided p values < 0.05 were considered significant. *p < 0.05; **p < 0.01. Source data, including exact p values, are provided as a Source data file.