Fig. 7. Anti-IL-10 neutralizing antibody increased cDNT cytotoxicity toward M2 macrophages in vivo.

a Representative H&E staining and oil red o staining in liver paraffin sections of mice fed with MCD diet for 4 weeks. Scale bars, 100 μm (n = 6 biologically independent samples per group). b Statistical analysis of NAS score and positive area of oil red o staining in each group. W/O cDNT, red; W/ cDNT, blue; W/ cDNT+anti-IL-10, green. Actual p values (left → right): 0.0027, 0.022, 2.03e−7, 0.0037. c Plasma ALT, AST, and TG levels were measured. Actual p values (left → right): 0.000002, 0.0039, 0.00077, 6.81e−9. d Representative flow cytometry plots of hepatic macrophages in MCD-fed mice. e, f Statistical analysis of the percentages of macrophages (e), M1 and M2 macrophages (f) among CD45.2⁺ cells in liver tissue. Actual p values (left → right): e 0.00042, 0.048; f 0.000017, 0.0011. g, h The Annexin V (g) and Ki67 staining (h) of hepatic M1 and M2 macrophages in MCD-fed mice with or without anti-IL-10 administration for 4 weeks. Actual p values (left → right): g 0.0060, 0.0018; h 0.000032, 0.000023. i, j Bcl-2 and Bcl-xl expression levels in intrahepatic M1 and M2 macrophages determined by flow cytometry, and relative changes of MFI from each group were determined. Actual p values (left → right): i 0.017, 0.0095; j 0.00023, 0.049. k Relative changes of NKG2A and NKG2D expression in CD45.1⁺ cDNT in MCD-fed mice with or without anti-IL-10 treatment. **p = 0.002. Data are presented as the mean ± SD; n = 6 mice/group. Statistical analysis for the first left figure in b (NAS score group) was performed by Kruskal–Wallis multiple comparisons test, statistical analysis for k was performed by Student’s t test, and others were performed by one-way ANOVA with post hoc multiple comparisons test. Two-sided p values < 0.05 were considered significant. *p < 0.05; **p < 0.01. Source data, including exact p values, are provided as a Source data file.