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. 2021 Jan 15;8:619914. doi: 10.3389/fbioe.2020.619914

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Copyright © 2021 Aprile and Kelly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The inhibitory effect of early HP stimulation on chondrogenesis of MSCs. Schematics depicting (A) the sample preparation procedure and (B) the experimental design. (C) Qualitative analysis of MSC viability after 7 days of dynamic culture. Live cells stained in green, dead cells in red. Scale bar 100 μm. (D) Chondrogenic gene expression levels relative to the SOFT FS group. MSCs were cultured for 7 days in presence of chondrogenic factors and HP stimulation. FS, Free Swelling group; HP, Hydrostatic Pressure group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.