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. 2021 Jan 15;8:619914. doi: 10.3389/fbioe.2020.619914

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Copyright © 2021 Aprile and Kelly.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The influence of HP on nuclear SMAD 2/3 and NCAD levels in MSCs cultured within soft and stiff IPN hydrogels. Confocal analysis of MSCs cultured for 7 days in presence of TGF-β3+HP. Cells stained for nuclei (blue), actin (magenta) and (A) SMAD 2/3 (gray) or (B) NCAD (cyan). Scale bars, 10 μm. Quantitative analysis of confocal images to determine the fold change of (A) the nuclear content of SMAD 2/3 and (B) NCAD expression. *p < 0.05, **p < 0.01, ****p < 0.0001.