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Delayed exposure to HP enhances chondrogenesis. (A) Gene expression analysis of MSCs cultured for 1 week in presence of TGF-β3 followed by 1 week of HP and TGF-β3. (B) Graphs reporting single cell volume after 14 days of culture. (C) Representative images of cell aggregates and (D) their quantification. Cells stained for actin (magenta). Scale bar 100 μm, *p < 0.05, **p < 0.01, ****p < 0.0001.
