Table 2.

List of specific primers for real time PCR.

Gene name	Gene full name	Forward/Reverse	T(°C) in use	T(°C) predicted	Gene ID	Gen bank no.
B2M	Beta-2-microglobulin	F:5′ ACTGAGTTCACTCCTAACG 3′	60	54.2	397033	NM_213978
		R:5′ TGCAGCATCTTCATAATCTC 3′		58
18S	Ribosomal protein S18	F:5′ CAACACCACATGAGCATATC 3′	60	59	396980	NM_213940
		R:5′ AGAAGTTCCAGCACATTTTG 3′		59.4
ACAN	Aggrecan	F:5′ CACCCCATGCAATTTGAG 3′	60	62.6	397255	NM_001164652
		R:5′ AGATCATCACCACACAGTC 3′		55.5
SOX9	SRY-box 9	F:5′ GACTGCTGAATGAGAGCGAGA 3′	60	59.87	396840	NM_213843.2
		R:5′ GAAGATGGCGTTGGGAGAGAT 3′		59.86
CDH2	Cadherin 2	F:5′ AGGTTTGCCAGTGTGACTCC 3′	60	60.18	100515322	XM_021096205.1
		R:5′ TCTCGGCGCTTCATCCATAC 3′		59.97
COL1	Collagen type I alpha 1 chain	F:5′ TAGACATGTTCAGCTTTGTG 3′	60	56.4	100738123	XM_021067153
		R:5′ GTGGGATGTCTTCTTCTTG 3′		57.1
COL2	Collagen type II alpha 1 chain	F:5′ CGACGACATAATCTGTGAAG 3′	60	58.3	397323	XM_001925959
		R:5′ TCCTTTGGGTCCTACAATATC 3′		59.3
COLX	Collagen type X alpha 1 chain	F:5′ CCAACATCCAGAATCCATC 3′	60	60.04	448809	NM_001005153
		R:5′ GTAGGTGTTTGGTATTGCTC 3′		59.3
RUNX2	Runt related transcription factor 2	F:5′ CCAACAGAGGCATTTAAGG 3′	60	59.7	100155806	XM_003482202
		R:5′ CCAAAAGAAGTTTTGCTGAC 3′		59