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. 2021 Jan 15;8:615850. doi: 10.3389/fbioe.2020.615850

FIGURE 5.

FIGURE 5

FOXO3 overexpression reversed the protective effects of hucMSC-exo in H/R injury. (A) FOXO3 was successfully overexpressed in AC16 cells. (B–F) AC16 cells overexpressing FOXO3 or vector were incubated with hucMSC-exo for 24 h, followed by H/R. LDH release (B), pyroptosis rate (C, Hoechst 33342/PI staining, Scale bar: 100 μm), protein expression of NLPR3, activated caspase-1(p20), pro-Caspase-1, and GSDMD-N (D,E, Western blot analysis), and the release of IL-1β and IL-18 (F, ELISA analysis) were determined. ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control + H/R; ++P < 0.01, +++P < 0.001 vs. vector + exo.