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. 2021 Jan 29;19(8):1726–1729.e3. doi: 10.1016/j.cgh.2021.01.044

Figure 1.

Figure 1

Microvascular pathology and regenerative responses in livers of COVID-19 patients. SARS-CoV-2 replication, histopathology, and regeneration responses of the liver in fatal COVID-19. (A) Viral replication. SARS-CoV-2 viral RNA (“viral RNA”; +strand, brown dots indicated by black arrows) detected by in situ hybridization and replicative viral intermediates (“viral replication”; -RNA, brown dots indicated by black arrows). Immunohistochemistry shows immunoreactivity against viral nucleocapsid protein (red) in hepatocytes within acinar zones I and II, periportal HSPC, cholangiocytes of portal bile ducts, and premature hepatocytes along the canal of Hering. (B) Microvascular pathology. Sinusoidal platelet aggregates and thrombi (anti-CD42b staining), frequent in COVID-19 patients. Sinusoidal dilatation is noted in hematoxylin and eosin (HE) staining. Coherent Raman scattering microscopy provided label-free contrast for tissue structure (grey) and lipid droplet identification (yellow), whereas second harmonic generation provided contrast for collagen fibers (blue). (C) Liver injury. High levels of hepatocyte steatosis in COVID-19 patients, in comparison with control subjects, as detected by HE staining and coherent Raman scattering/second harmonic generation (lipid droplets in yellow and collagen in blue). White arrows indicate macrovesicular steatosis, whereas black arrows show microvesicular steatosis. (D) Intrahepatic stem cell niche. Drawing of the bipotent intrahepatic stem cell compartment, which is located in the canal of Hering. On severe hepatic injury HSPC emerge and expand, contributing to liver repair. HSPC are detected as single cells (indicated by black arrows) or in regenerative clusters comprised of EpCAM+ HSPC, hepatobiliary intermediate cells, and premature hepatocytes, which are adjacent to the canal of Hering. Stem cell marker EpCam (red) is also expressed by numerous HSPC and intermediates of hepatocytes/cholangiocytes and regenerative clusters of periportal hepatocytes of irregular size and shape. HSPC frequency in column diagram represents numbers of HSPC per 10 field views. ∗P < .05.