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. 2020 Aug 25;126(1):245–255. doi: 10.1016/j.bja.2020.07.022

Figure 5.

Figure 5

Oscillations in CeM neurones after injections of 3β-OH. (a) Total δ power after injections of the vehicle 2-hydroxypropyl-β-cyclodextrin (Day 1) and 3β-OH (Day 2) in WT and Cav3.1 KO mice. Mutant mice had less δ power on Day 1 (two-way RM analysis of variance [anova]: interaction F(11,121)=0.27, P=0.990; time F(11,121)=1.15, P=0.330; mutation effect F(1,11)=5.93, P=0.033). 3β-OH enhanced δ power in WT animals (two-way RM anova: interaction F(11,121)=1.51, P=0.137; time F(11,121)=3.62, P<0.001; mutation effect F(1,11)=11.99, P=0.005). (b) Total θ power after vehicle (Day 1) or 3β-OH (Day 2) in WT and Cav3.1 KO mice. 3β-OH enhanced θ power in WT animals (two-way RM anova: interaction F(11,121)=4.07, P<0.001; time F(11,121)=4.03, P<0.001; mutation effect F(1,11)=26.60, P<0.001; Sidak's post hoc presented in figure). (c) Total α power for vehicle (Day 1) or 3β-OH (Day 2) in WT and Cav3.1 KO mice. 3β-OH enhanced α power in WT animals (two-way RM anova: interaction F(11,121)=5.87, P<0.001; time F(11,121)=5.10, P<0.001; mutation effect F(1,11)=27.74, P<0.001; Sidak's post hoc presented in figure). (d) Total β power for vehicle (Day 1) or 3β-OH (Day 2) in WT and Cav3.1 KO mice. 3β-OH enhanced β power in WT animals (two-way RM anova: interaction F(11,121)=1.16, P=0.324; time F(11,121)=2.82, P=0.003; mutation effect F(1,11)=8.62, P=0.013; Sidak's post hoc presented in figure). (e) Total γ power for vehicle (Day 1) or 3β-OH (Day 2) in WT and Cav3.1 KO mice. Mutant mice had less γ power on Day 1 (two-way RM anova: interaction F(11,121)=0.53, P=0.879; time F(11,121)=1.77, P=0.067; mutation effect F(1,11)=6.73, P=0.025). 3β-OH did not statistically change γ power between WT and Cav3.1 KO animals. WT, seven animals, Cav3.1 KO, six animals; ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001. 3β-OH, (3β,5β,17β)-3-hydroxyandrostane-17-carbonitrile; CeM, central medial nucleus; KO, knock-out; LFP, local field potentials; WT, wild-type.