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(A) Alizarin red staining of the hAD-MSCs from four donors differentiated in the presence of 2.5% hPL, 10% HS, or 10% FCS. The influence of cell culture supplements on the calcium deposition evaluated by Alizarin red extraction, 10× objective, scale bar 100 μm. (B) and on the cell viability during the differentiation (C). Data represent the mean ± SD of a threefold determination for four donors.
