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(A) Alkaline phosphatase staining of differentiated hAD-MSCs cultivated for 7, 14, and 21 days and differentiated in medium supplemented with FCS, HS and hPL, 10× objective, scale bar 100 μm. (B) Measured ALP activity in the supernatant of hAD-MSCs cultivated with FCS, HS and hPL for 7, 14, and 21 days. Data represent the mean ± SD of a threefold determination for two donors.
