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. 2021 Jan 15;8:598389. doi: 10.3389/fbioe.2020.598389

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Copyright © 2021 Kirsch, Rach, Handke, Seltsam, Pepelanova, Strauß, Vogt, Scheper and Lavrentieva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The influence of cell culture supplements on the calcium deposition evaluated by using a trained metric phase object confluence mask for microscopic pictures analyzed by the IncuCyte Live-Cell Imaging System and Software (Essen BioScience, Ann Arbor, MI). Data represent the mean ± SD for a fourfold determination. (B) Microscopic pictures taken by an IncuCyte Live-Cell Imaging System and blended with a trained metric phase object confluence mask, which indicates typical osteogenic changes of the cells, scale bar 400 μm.