Table 3. Unloaded single-molecule motility properties.
| KIF1A motor | Velocity (μm/s)a | Run length (μm)b | Landing rate (events⋅min−1⋅nM−1⋅μm−1)c |
|---|---|---|---|
| KIF1A(1–393)C | 2.1 ± 0.1 | 16.7 (10.2, 27.2) | 10.7 ± 0.6 |
| KIF1A(1–393)-V8MC | 1.3 ± 0.1e | 4.1 (2.1, 7.0)f | 1.5 ± 0.1e |
| KIF1A(1–393)-Y89DC | 1.7 ± 0.1e | 2.0 (1.2, 3.5)f | 2.8 ± 0.1e |
| KIF1A(1–393)-WTE | 2.5 ± 0.2 | 12.2 (6.7, 18.4) | ND |
| KIF1A(1–393)-V8ME | 1.3 ± 0.1 | 7.3 (4.4, 12.2) | ND |
| KIF1A(1–393)-Y89DE | 1.7 ± 0.2 | 6.3 (4.0, 10.7) | ND |
| KIF1A(FL)C | 0.3 ± 0.1 | 2.8 (1.5, 3.7) | 2.0 ± 0.2 |
| KIF1A(FLAct)C | 1.9 ± 0.1 | 9.3 (4.7, 12.7) | 2.8 ± 0.6 |
| KIF1A(FLAct)-V8MC | 1.1 ± 0.1d | 8.0 (4.0, 10.6)g | 5.1 ± 0.4d |
| KIF1A(FLAct)-Y89DC | 1.4 ± 0.1d | 8.5 (4.5, 11.8)h | 5.9 ± 0.4d |
a
Data are reported as mean ± SEM.
b
Data are reported as median (quartiles).
c
Includes diffusive + processive events.
d
P < 0.001 (Student’s t test) compared with KIF1A(FLAct)C.
e
P < 0.001 (Student’s t test) compared with KIF1A(1-393)C.
f
P < 0.001 (Kruskal-Wallis test) compared with KIF1A(1-393)C.
g
P = 0.027 (Kruskal-Wallis test) compared with KIF1A(FLAct)C.
h
P = 0.38 (Kruskal-Wallis test) compared with KIF1A(FLAct)C.