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. 2021 Jan 26;218(3):e20201915. doi: 10.1084/jem.20201915

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© 2021 Yang et al.

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Figure S1. — Vancomycin decreased Lachnospiraceae in the gastrointestinal tract, while both Lachnospiraceae supplementation and butyrate administration impaired the antitumor effect of IR, in a melanoma model. (A) Verification of the abundance of Lachnospiraceae by qPCR using Lachnospiraceae (TSD-26) genomic DNA as a standard (n = 4/group). Unpaired t tests were used to analyze the data. (B) GF C57BL/6 mice (n = 5/group) were fed Lachnospiraceae and K. alysoides (TSD-26) for 1 mo, 10⁸ CFU/mouse weekly. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10⁶ B16F1 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. (C) C57BL/6 mice (n = 5/group) were injected s.c. with 10⁶ B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were injected i.v. with 4 μmol of NaBu on days 1, 4, and 7 after IR. (D) C57BL/6 mice (n = 5/group) were injected s.c. with 10⁶ B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were intratumorally (i.t.) injected with 2 μmol of NaBu on days 1, 4, and 7 after IR. A tumor growth curve is shown. Two-way ANOVA tests were used to analyze the tumor growth data. *, P < 0.05; ****, P < 0.0001. One representative experiment (out of two experiments) is shown.