Table 2. Comparisons of fresh and post-thaw microscopic parameters of normal motility semen samples, frozen with TEST Yolk Buffer or ANTIOX-PC cryopreservation medium.
| Variable | Fresh sêmen | Post-thaw | P value | ||||
|---|---|---|---|---|---|---|---|
| TYB | ANTIOX-PC | F × TYB | F × ANTIOX-PC | TYB × ANTIOX-PC | |||
| Concentration (106/mL) | 125.19±92.03 | 104.32±72.55 | 97.40±69.98 | <0.0001 | <0.0001 | 0.0951 | |
| Morphology (%) | 2.30±1.07 | 1.54±0.62 | 1.40±0.52 | <0.0001 | <0.0001 | 0.1753 | |
| Vitality (%) | 84.76±6.93 | 39.22±10.05 | 36.65±10.64 | <0.0001 | <0.0001 | 0.1484 | |
| Progressive (%) | 47.37±9.55 | 12.41±11.53 | 12.54±11.32 | <0.0001 | <0.0001 | 0.9082 | |
| Nonprogressive (%) | 30.56±7.59 | 22.92±10.39 | 26.94±11.43 | <0.0001 | 0.0160 | 0.0076 | |
| Immotile (%) | 22.08±8.13 | 64.67±17.20 | 60.87±17.07 | <0.0001 | <0.0001 | 0.0435 | |
| Total motility (%) | 77.92±8.13 | 35.33±17.20 | 39.48±17.29 | <0.0001 | <0.0001 | 0.0283 | |
| DFI (%) | 7.71±7.75 | 15.59±10.86 | 16.97±11.29 | <0.0001 | <0.0001 | 0.3030 | |
Values are mean ± standard deviation. TYB, TEST Yolk Buffer cryoprotectant; ANTIOX-PC, synthetic cryoprotectant supplemented with phosphatidylcholine and antioxidant L-acetyl-carnitine; F, Fresh semen; DFI, DNA fragmentation index. α=0.05.