Table 3. Fresh and post-thaw microscopic parameters of low motility semen samples frozen with TEST Yolk Buffer or ANTIOX-PC freezing medium.
| Variable | Fresh sêmen | Post-thaw | P value | ||||
|---|---|---|---|---|---|---|---|
| TYB | ANTIOX-PC | F × TYB | F × ANTIOX-PC | TYB × ANTIOX-PC | |||
| Concentration (106/mL) | 57.61±40.37 | 16.56±13.01 | 13.88±12.90 | <0.0001 | <0.0001 | 0.5338 | |
| Morphology (%) | 2.33±1.68 | 2.28±1.66 | 2.29±1.75 | 0.6371 | 0.7531 | 0.8750 | |
| Vitality (%) | 76.55±11.92 | 45.81±20.60 | 48.71±26.26 | <0.0001 | <0.0001 | 0.1185 | |
| Progressive (%) | 17.88±8.46 | 12.09±11.11 | 12.40±11.49 | <0.0001 | <0.0001 | 0.7869 | |
| Nonprogressive (%) | 43.45±12.09 | 22.71±13.84 | 30.97±18.46 | <0.0001 | <0.0001 | <0.0001 | |
| Immotile (%) | 38.67±16.34 | 65.00±23.00 | 56.64±26.77 | <0.0001 | <0.0001 | <0.0001 | |
| Total motility (%) | 61.33±16.34 | 34.79±22.86 | 43.36±26.77 | <0.0001 | <0.0001 | <0.0001 | |
| DFI (%) | 9.03±7.69 | 15.83±11.04 | 13.33±10.74 | <0.0001 | 0.0023 | 0.0742 | |
Values are mean ± standard deviation. TYB, TEST Yolk Buffer cryoprotectant; ANTIOX-PC, synthetic cryoprotectant supplemented with phosphatidylcholine and antioxidant L-acetyl-carnitine; F, Fresh semen; DFI, DNA fragmentation index. α=0.05.