Table 1. Gene ontology analysis of common upregulated and downregulated DEGs.
| Category | Term | Count | Gene ratio | P value |
|---|---|---|---|---|
| Upregulated | ||||
| BP | GO:0030198~extracellular matrix organization | 6 | 0.0775294 | 2.59E-04 |
| BP | GO:0008285~negative regulation of cell proliferation | 6 | 0.0775294 | 0.005787 |
| BP | GO:0007155~cell adhesion | 6 | 0.0775294 | 0.0105986 |
| BP | GO:0044267~cellular protein metabolic process | 5 | 0.0646078 | 3.85E-04 |
| BP | GO:0043066~negative regulation of apoptotic process | 5 | 0.0646078 | 0.04328502 |
| CC | GO:0070062~extracellular exosome | 25 | 0.3230392 | 5.98E-08 |
| CC | GO:0005615~extracellular space | 20 | 0.2584313 | 9.39E-10 |
| CC | GO:0005576~extracellular region | 18 | 0.2325882 | 6.52E-07 |
| CC | GO:0031012~extracellular matrix | 11 | 0.1421372 | 6.35E-09 |
| CC | GO:0005578~proteinaceous extracellular matrix | 6 | 0.0775294 | 8.18E-04 |
| MF | GO:0005102~receptor binding | 6 | 0.0775294 | 0.00290085 |
| MF | GO:0005509~calcium ion binding | 6 | 0.0775294 | 0.04816598 |
| MF | GO:0005518~collagen binding | 5 | 0.0646078 | 2.29E-05 |
| MF | GO:0008201~heparin binding | 5 | 0.0646078 | 0.00100954 |
| MF | GO:0017147~Wnt-protein binding | 3 | 0.0387647 | 0.00335112 |
| Downregulated | ||||
| BP | GO:0007283~spermatogenesis | 68 | 0.0747565 | 1.86E-33 |
| BP | GO:0007275~multicellular organism development | 50 | 0.054968 | 2.82E-13 |
| BP | GO:0030154~cell differentiation | 44 | 0.0483718 | 1.24E-11 |
| BP | GO:0007286~spermatid development | 24 | 0.0263846 | 1.21E-17 |
| BP | GO:0030317~sperm motility | 21 | 0.0230866 | 1.32E-17 |
| CC | GO:0005634~nucleus | 221 | 0.2429586 | 3.59E-05 |
| CC | GO:0005737~cytoplasm | 197 | 0.216574 | 0.00769222 |
| CC | GO:0005813~centrosome | 29 | 0.0318814 | 3.33E-04 |
| CC | GO:0031514~motile cilium | 25 | 0.027484 | 8.08E-18 |
| CC | GO:0001669~acrosomal vesicle | 24 | 0.0263846 | 8.40E-16 |
| MF | GO:0000287~magnesium ion binding | 12 | 0.0131923 | 0.0461689 |
| MF | GO:0004722~protein serine/threonine phosphatase activity | 7 | 0.0076955 | 0.00798661 |
| MF | GO:0003796~lysozyme activity | 6 | 0.0065962 | 1.66E-05 |
| MF | GO:0004674~protein serine/threonine kinase activity | 18 | 0.0197885 | 0.06625263 |
| MF | GO:0003777~microtubule motor activity | 6 | 0.0065962 | 0.09501651 |
GO, gene ontology; DEGs, differentially expressed genes; BP, biological process; CC, cellular component; MF, molecular function; Count, number of DEGs.