FIG 4.

Effect of Env-A539V and RT-Y188L mutations on susceptibility to EFV. (A) The SupT1 T-cell line was transfected with the indicated proviral clones in the absence or presence of 1,000 nM EFV. Virus replication kinetics were monitored by measuring RT activity at the indicated time points. Data are representative of three independent experiments. (B) SupT1 T cells were transfected with the indicated proviral clones in the absence or presence of serial dilutions of EFV (0.03 to 1,000 nM). The dose-dependent inhibition curve was determined based on RT values at the peak of multicycle spreading virus replication. (C) TZM-bl cells were exposed to 100 TCID₅₀ of the indicated viruses in the absence or presence of serial dilutions of EFV (0.1 to 10,000 nM). Luciferase activity was measured at 48 h postinfection. (D) IC₅₀ values for EFV in multicycle spreading and cell-free infection based on the data in panels B and C. Data from at least three independent experiments are shown as means ± SEs. Statistical analysis by unpaired t test.