FIG 9.

The sensitivity of NL(AD8) Env-N654K to NAbs recognizing different Env conformations, anti-CD4 Ab, and entry inhibitors. TZM-bl cells were infected with 100 TCID₅₀ of the indicated viruses in the presence of various concentrations of NAbs (A to G), sCD4 (H), anti-CD4 Ab SIM.4 (I), and entry inhibitors (J to L). Luciferase activity was measured at 48 h postinfection. Data from at least three independent experiments are shown as means ± SEs. ***, P < 0.001; **, P < 0.01; *, P < 0.05 by unpaired t test. The asterisks for 0 h or 2 h incubation with sCD4 (H) are indicated as black or gray, respectively.