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RBMS3 prevented the Wnt/β-catenin pathway in breast cancer cells. MCF-7 cells were transfected with RBMS3-pcDNA3.1 or pcDNA3.1. (A) The expression of β-catenin, c-Myc, and cyclin D1 was evaluated by Western blot. The relative expression of each gene was normalized to the housekeeping gene GAPDH. (B) Quantification analysis of relative protein levels of β-catenin, c-Myc, and cyclin D1 was performed using Gel-Pro Analyzer version 4.0 software. *p < 0.05 compared to the pcDNA3.1 group.
