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. 2018 Jan 19;26(1):111–122. doi: 10.3727/096504017X14924753593574

Figure 5.

Figure 5

Knockdown of CUL3 expression decreased cellular proliferation of NPC by modulating the cell cycle. (a) Interference efficiency of CUL3 knockdown in CNE-2 cells (CNE-2, cells with no transfection; sh-NC, transfected with scrambled shRNA; CUL3-sh, transfected with CUL3-shRNA-encoding lentivirus) detected by real-time RT-PCR analysis. Highest interference efficiency of CUL3 knockdown was observed in cells transduced with CUL3-shRNA2 (CUL3-sh2). *p < 0.05 versus the sh-NC group. (b) Proliferation of CNE-2 cells after CUL3 knockdown using CUL3-sh2 was assessed by the cell counting kit-8 (CCK8) assay. *p < 0.05 versus the sh-NC group. (c) Colony formation assay in CNE-2 cells with depletion of CUL3 expression. *p < 0.05 versus the sh-NC group. (d) Cell cycle analysis in CNE-2 cells after depletion of CUL3 expression. Data are presented as mean ± SD. The same experiment was conducted in triplicate. *^p < 0.05 versus the sh-NC group. (e) Western blotting analysis of CUL3, and the PCNA expression in CUL3 knockdown CNE-2 cells and in CUL3 overexpression HNE-2 cells. *^&#p < 0.05 versus the sh-NC and LV-NC groups.