Expression of the cathepsin F (CTSF) gene in gastric carcinoma cell lines and a gastric epithelial cell line. (A) CTSF mRNA was detectable at different levels in gastric carcinoma cell lines (SGC7901, BGC823, MKN45, HGC27, AGS, and MGC803) and a gastric epithelial cell line (GES1). (B) Cell lysate was loaded and probed with anti-CTSF antibody (60 kDa) with GAPDH (36 kDa) as an internal control. CTSF protein expression was comparatively higher in SGC7901, BGC823, and MKN45 cells than in other gastric cancer (GC) cell lines (**p < 0.01). Results are representative of three different experiments, and data are expressed as mean ± standard deviation.