Figure 3.

Transfection efficiency tested by GFP-Lenti-shRNA under a fluorescence microscope and knockdown efficiency examined by qPCR. (A, C, E) Fluorescent particles within the cells indicated that Lenti-shRNA was successfully transfected into GC cells. (B, D, F) qPCR results showed that KD-1 has a higher efficiency of silencing CTSF expression than the other two shRNAs (KD-2 and KD-3). The ratio of CTSF knockdown was compared in the three groups (KD-1, KD-2, and KD-3). Each bar indicates the mean silencing rate ± standard deviation per group. All experiments were done three times, and data are presented as mean ± SD. **p < 0.01. (B) Visualization of the cells under common light. (G) Visualization of the cells under GFP (green fluorescent protein) fluorescence light. Lenti-shRNA, lentivirus-mediated small hairpin RNA; KD-1, Lenti-shRNA1; KD-2, Lenti-shRNA2; KD-3, Lenti-shRNA3. Magnification: ×200.