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CircANKIB1 was overexpressed in OS tissues and cells. (A) The expression of circANKIB1 was detected by qRT-PCR in OS tissues (n = 35) and adjacent normal tissues (n = 35). (B) The expression of circANKIB1 was measured by qRT-PCR in OS cells (U2OS and HOS) and hFOB cells. (C and D) The expression levels of circANKIB1 and ANKIB1 mRNA were determined after treatment with Actinomycin D and RNase R by qRT-PCR in U2OS and HOS cells. *P < 0.05.
