Fig. 2.

CircANKIB1 knockdown inhibited cell proliferation, migration and invasion, and accelerated apoptosis in OS cells. U2OS and HOS cells were transfected with si-NC or si-circANKIB1. (A) Knockdown efficiency of circANKIB1 was determined by qRT-PCR. (B) Colony formation assay was performed to examine the number of colonies. (C and D) Flow cytometry analysis was used to detect cell cycle distribution and apoptosis rate. (E) Wound healing assay was utilized to assess cell migration ability (×40). (F and G) Transwell assay was employed to evaluate cell migration and invasion abilities (×100). (H and I) Western blot assay was carried out to measure the expression levels of E-cadherin and Vimentin. *P < 0.05.