Fig. 4.

Silence of circANKIB1 suppressed the progression of OS cells by sponging miR-217. (A) The knockdown efficiency of miR-217 was examined by qRT-PCR in U2OS and HOS cells transfected with miR-NC inhibitor or miR-217 inhibitor. (B-I) U2OS and HOS cells were transfected with si-NC, si-circANKIB1, si-circANKIB1 + miR-NC inhibitor, or si-circANKIB1 + miR-217 inhibitor. (B) Colony formation assay was used to examine colony formation ability. (C and D) Cell cycle distribution and cell apoptosis rate were determined by flow cytometry analysis. (E-G) Cell migration and invasion were measured by wound healing assay and transwell assay. (H and I) Western blot assay was performed to test the protein levels of E-cadherin and Vimentin. *P < 0.05.