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. 2021 Jan 14;27:100347. doi: 10.1016/j.jbo.2021.100347

Fig. 5.

Fig. 5

PAX3 was a target gene of miR-217 in OS cells. (A) PAX3 had binding sites for miR-217. (B) Relative luciferase activity was measured in U2OS and HOS cells co-transfected with WT-PAX3 or MUT-PAX3 and miR-217 mimic or miR-NC mimic. (C) Overexpression efficiency of miR-217 was determined by qRT-PCR in U2OS and HOS cells transfected with miR-NC mimic or miR-217 mimic. (D) The protein expression of PAX3 was tested by western blot analysis in U2OS and HOS cells transfected with miR-NC inhibitor, miR-217 inhibitor, miR-NC mimic, or miR-217 mimic. (E and F) PAX3 mRNA and protein expression were detected in OS tissues and adjacent normal tissues by qRT-PCR and western blot analyses, respectively. (G) Western blot sassy was conducted to measure the protein expression of PAX3 in OS cells (U2OS and HOS) and hFOB cells. (H) The correlation between PAX3 mRNA expression and miR-217 expression was analyzed in OS tissues. *P < 0.05.