Fig. 8.

CircANKIB1 downregulation suppressed tumor growth via regulating miR-217 and PAX3 in vivo. Sh-NC or sh-circANKIB1-transfected HOS cells were subcutaneously injected into BALB/c nude mice to establish mice xenograft model. (A) Tumor volume was analyzed every week in mice. (B) Tumor weight was calculated after injection for 4 weeks. (C and D) The expression levels of circANKIB1 and miR-217 in tumor tissues were detected by qRT-PCR. (E) The protein expression of PAX3 in tumor tissues was measured by western blot assay. *P < 0.05.