Long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) was highly expressed in lung cancer cell lines. The expression levels of lncRNA UCA1 in human normal embryonic lung WI-38, HEL-1 cells, and lung cancer A549, H157, H4006, H1299, and H1650 cells were detected by qualitative reverse transcriptase polymerase chain reaction (qRT-PCR) assay. Data were expressed as means ± standard deviation (SD) of three independent experiments. *p < 0.05 versus WI-38 cells; #p < 0.05 or ##p < 0.01 versus HEL-1 cells.