Figure 2.

Knockdown of lncRNA UCA1 inhibited cell viability, migration, invasion, and cell cycle progression, but promoted cell apoptosis. After transfection with short hairpin RNA (sh)-UCA1 and negative control (NC) in A549 cells, the (A) expression levels of lncRNA UCA1, (B) cell viability, (C) relative migration, (D) relative invasion, (E) cell apoptosis, (F) expression levels of B-cell lymphoma-2 (Bcl-2), Bcl2-associated X (Bax), caspase 3, caspase 9, and (G) expression levels of proliferating cell nuclear antigen (PCNA), cyclin A, cyclin E1, cyclin D1, cyclin-dependent kinase (CDK2), and CDK4 were measured by qRT-PCR assay, trypan blue exclusion assay, Transwell assay, flow cytometry analysis, and Western blotting, respectively. Data were expressed as means ± SD of three independent experiments. *p < 0.05.