Figure 6.

Different expressions of PBX3 have an influence on A549 cell viability, migration, invasion, apoptosis, and cycle transition. A549 cells were transfected with pEX-PBX3 or sh-PBX3. (A, B) mRNA expression of PBX3, (C) viability, (D) migration, (E) invasion, (F) apoptosis, (G) protein expression of Bcl-2, Bax, caspase 3, and caspase 9, (H) protein expression of PCNA, cyclin A, cyclin E1, cyclin D1, CDK2, and CDK4 was analyzed using qRT-PCR assay, trypan blue exclusion assay, Transwell assay, flow cytometry analysis, and Western blotting, respectively. Data were expressed as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.