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. 2018 Jun 11;26(5):691–701. doi: 10.3727/096504017X15101398724809

Figure 1.

Figure 1

Trophinin-associated protein (TROAP) is downregulated in hepatocellular cancer (HCC) tissues and cell lines. (A) The mRNA levels of TROAP from 52 pairs of HCC tissues and matched noncancerous tissues were tested using quantitative reverse transcription polymerase chain reaction (qRT-PCR). ***p < 0.001. (B) The protein levels of TROAP in 12 pairs of HCC tissues and matched noncancerous tissues were tested by Western blotting. N, non-cancerous; T, tumor. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. The mRNA and protein expression levels of TROAP in one immortalized hepatic cell line (LO2) and seven liver cancer cell lines (97L, HepG2, Hep3B, Huh7, SMMC-7721, BEL-7404, and QGY-7703) were determined by qRT-PCR (C) and Western blotting (D), respectively. Data of qRT-PCR were presented as mean ± standard deviation (SD).