Skip to main content
. 2018 Apr 10;26(3):411–420. doi: 10.3727/096504017X14974343584989

Figure 3.

Figure 3

Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) is a direct target of miR-139-5p in OC cells. (A) The putative miR-139-5p binding sites and mutant (Mut) 3′-untranslated region (3′-UTR) ROCK2 sites are shown. The replaced site is underlined. WT, wild type; MT, mutant type. (B) Relative luciferase activity was measured in SKOV3 cells cotransfected with WT/MT-ROCK2-3′UTR reporter plasmid and miR-139-5p mimic or miR-NC. (C, D) The ROCK2 expression on mRNA and protein levels was measured in SKOV3 cells transfected with miR-139-5p mimic and miR-NC. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. **p < 0.01.