Relative expression of microRNA-744 (miR-744) is downregulated in colorectal cancer (CRC) tissues and cell lines. (A) Total RNA was extracted from 53 paired CRC tissues and adjacent nontumor tissues, and subjected to reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis for the detection of miR-744 expression. *p < 0.05 versus nontumor tissues. (B) RT-qPCR analysis was performed to measure miR-744 expression in five human CRC cell lines (LoVo, SW480, SW620, HCT116, and HT29) and a normal human colon epithelium cell line (FHC). *p < 0.05 versus FHC.