Skip to main content
. 2018 Oct 17;26(9):1401–1409. doi: 10.3727/096504018X15188747585738

Figure 3.

Figure 3

Notch1 is a direct target of miR-744 in CRC. (A) Predicted and mutated miR-744 binding sequences in the 3′-untranslated region (3′-UTR) of Notch1. (B) SW480 and HCT116 cells were cotransfected with miR-744 mimic or miR-NC and p-MIR-WT-Notch1-3′-UTR or p-MIR-MUT-Notch1-3′-UTR. At 48 h after transfection, cells were harvested and subjected to the analysis of luciferase activity using the Dual-Luciferase Reporter Assay System. *p < 0.05 versus miR-NC. miR-744 mimics or miR-NC was transfected into SW480 and HCT116 cells. (C) RT-qPCR and (D) Western blot analysis were applied to measure Notch1 mRNA and protein levels, respectively. *p < 0.05 versus miR-NC.