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. 2018 Sep 14;26(8):1201–1205. doi: 10.3727/096504018X15154104709325

Figure 1.

Figure 1

ETS-translocation variant 6 (ETV6)/FMS-like tyrosine kinase-3 (FLT3) could coimmunoprecipitate with heat shock protein 90 (Hsp90) in 293FT, cos7, and HeLa cells transiently expressing ETV6/FLT3. 293FT, cos7, and HeLa cells were transfected with the ETV6/FLT3 (EF-1) plasmids using Lipofectamine 2000 according to the manufacturer’s instructions. Cells were harvested after 48-h transfection. (A) Total cell lysates were tested for the expression of ETV6/FLT3 by immunoblotting (IB) using FLT3 (F-8) antibody. (B) Total cell lysates were immunoprecipitated with Hsp90 antibody. Precipitated proteins were subjected to IB analysis with FLT3 antibody and Hsp90 antibody. (C) Effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on the expression of ETV6/FLT3. cos7-EF1 cells were treated with or without 17-AAG (1 μM) for 5 h. Cell lysates were prepared and subjected to immunoprecipitation with Hsp90 antibody. Then IB was performed using FLT3 antibody to detect ETV6/FLT3 and Hsp90 antibody to detect total Hsp90.