Figure 2.
17-AAG accelerated the decay of ETV6/FLT3. 293FT, cos7, and HeLa cells were transfected with the EF-1 plasmids using Lipofectamine 2000 according to the manufacturer’s instructions. After 24 h of transfection, cells at a density of 1 × 105 cells/ml were treated with 17-AAG (1 μM) or cycloheximide (CHX; 100 μg/ml) or both of these agents or dimethyl sulfoxide (DMSO) alone as control for 2 h in cos7-EF-1 cells (A), in HeLa-EF-1 cells (B), and in 293FT-EF-1 cells (C). Total cell lysates were subjected to Western blot analysis with indicated antibodies. Relative expression levels of ETV6/FLT3, determined by densitometric analysis, are shown (lower rows).