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. 2018 Aug 23;26(7):987–996. doi: 10.3727/096504017X15140534417184

Figure 3.

Figure 3

V-crk avian sarcoma virus CT10 oncogene homolog-like (CRKL) is a direct target of miR-379 in cervical cancer. (A) Predicted miR-379 binding sites in the 3′-untranslated region (3′-UTR) of CRKL and its mutant containing altered nucleotides in the 3′-UTR. (B, C) HeLa and Ca-Ski cells were cotransfected with luciferase reporter plasmid and miR-379 mimics or miR-NC. Luciferase activities were detected 48 h after the transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. *p < 0.05 compared with miR-NC. RT-qPCR and Western blotting analysis were conducted to measure the mRNA (D) and protein (E) expression levels of CRKL in HeLa and Ca-Ski cells transfected with miR-379 mimics or miR-NC, respectively. *p < 0.05 compared with miR-NC.