Figure 2.

MG132 induced cell cycle arrest at the G₂/M phase and apoptosis in OS cells. MG-63 and HOS cells were treated with dimethyl sulfoxide (DMSO) or 10 μM MG132 for 24 h. (A, B) Cell cycle distribution was analyzed by flow cytometry. *p < 0.05 versus control (DMSO) denotes a significant difference. (C, D) Apoptotic rates of the treated cells were detected by flow cytometry. *p < 0.05 versus control (DMSO) denotes a significant difference. (E) Expression of PARP and p21^waf1 proteins in treated OS cells was detected by Western blot.