Figure 2.

CCAL knockdown suppressed cell proliferation. (A) RT-qPCR was used to determine the knockdown efficiency of CCAL in BCPAP and TPC-1 cells. (B–D) Cell counting kit-8 (CCK-8) and colony formation assays were used for the detection of cell proliferation potential in BCPAP and TPC-1 cells. (E) Cell cycle distribution of BCPAP and TPC-1 cells transfected with siCCAL relative to cells transfected with control was measured by propidium iodide staining using flow cytometry. *p < 0.05 and **p < 0.01 by two-tailed Student’s t-test. All data presented are shown as means ± SD collected from three independent experiments.