Figure 4.

CCAL knockdown inhibited tumor growth and metastasis in vivo. (A–C) BCPAP cells were injected into nude mice subcutaneously. Then tumor volumes (A) and tumor weight (B and C) were measured at indicative time points. (D) The numbers of metastatic foci in the lungs of mice from various groups at 8 weeks after tail vein injection were counted. (E) The protein levels of cyclin D1, Twist, and Snail were measured by Western blot. *p < 0.05 and **p < 0.01 by two-tailed Student’s t-test. All data presented are shown as means ± SD collected from three independent experiments.